產(chǎn)品編號(hào) | bsm-62586R |
英文名稱 | HNF4A Recombinant Rabbit mAb |
中文名稱 | 肝細(xì)胞核因子4α重組兔單抗 |
別 名 | HNF 4 alpha; HNF4; HNF4A; HNF4a7; HNF4a8; HNF4a9; Hnf4alpha; MODY; MODY1; NR2A1; NR2A21; TCF14; Tcf4. |
抗體來源 | Rabbit |
克隆類型 | Recombinant |
克 隆 號(hào) | 9C3 |
交叉反應(yīng) | Human |
產(chǎn)品應(yīng)用 | WB=1:500-1:2000,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | 53 kDa |
檢測(cè)分子量 | 53 |
性 狀 | Liquid |
免 疫 原 | A synthesized peptide derived from human HNF 4 alpha: 12-68 |
亞 型 | IgG |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 |
Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1-antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1-alpha. May be essential for development of the liver, kidney and intestine. SWISS: P41235 Gene ID: 3172 肝細(xì)胞核因子α(HNF4 α)是一類異質(zhì)的保守轉(zhuǎn)錄因子,參與調(diào)節(jié)肝臟內(nèi)糖代謝、脂代謝相關(guān)基因的表達(dá).HNF在胰島中也有表達(dá).HNF4-α目前用于2型糖尿病的研究也很多。 |
產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with HNF4A monoclonal antibody, unconjugated (bsm-62586R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (HNF4A) monoclonal Antibody, unconjugated (bsm-62586R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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