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Phospho-PBK (Thr9) Recombinant Rabbit mAb (bsm-62741R)  
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產(chǎn)品編號(hào) bsm-62741R
英文名稱 Phospho-PBK (Thr9) Recombinant Rabbit mAb
中文名稱 磷酸化PDZ連接激酶/T-LAK細(xì)胞源蛋白激酶重組兔單抗
別    名 CT84; MAPKK like protein kinase; Nori3; PBK; PDZ binding kinas; Serine/threonine protein kinase; SPK; TOPK.  
抗體來(lái)源 Rabbit
克隆類型 Recombinant
克 隆 號(hào)
交叉反應(yīng) Human
產(chǎn)品應(yīng)用 WB=1:500-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:100-1:200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 36 kDa
檢測(cè)分子量 40
性    狀 Liquid
免 疫 原 A synthesized peptide derived from human PBK around the phosphorylation site of T9: FK-pT-PS 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
保存條件 Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.

SWISS:
Q96KB5

Gene ID:
55872

PBK/TOPK是一種新近發(fā)現(xiàn)的絲-蘇氨酸激酶,屬于MAPKK分子家族成員,具有參與調(diào)控惡性腫瘤細(xì)胞的增殖和周期變化, 促進(jìn)腫瘤細(xì)胞轉(zhuǎn)化, 并且通過(guò)MAPKK信號(hào)通路參與調(diào)控細(xì)胞DNA損傷修復(fù).
產(chǎn)品圖片
Hela (H) cells were treated with or without Nocodazole, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-PBK (Thr9) monoclonal antibody, unconjugated (bsm-62741R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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