| 產(chǎn)品編號(hào) | bsm-62741R |
| 英文名稱 | Phospho-PBK (Thr9) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化PDZ連接激酶/T-LAK細(xì)胞源蛋白激酶重組兔單抗 |
| 別 名 | CT84; MAPKK like protein kinase; Nori3; PBK; PDZ binding kinas; Serine/threonine protein kinase; SPK; TOPK. |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:100-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36 kDa |
| 檢測(cè)分子量 | 40 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human PBK around the phosphorylation site of T9: FK-pT-PS |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. SWISS: Q96KB5 Gene ID: 55872 PBK/TOPK是一種新近發(fā)現(xiàn)的絲-蘇氨酸激酶,屬于MAPKK分子家族成員，具有參與調(diào)控惡性腫瘤細(xì)胞的增殖和周期變化, 促進(jìn)腫瘤細(xì)胞轉(zhuǎn)化, 并且通過(guò)MAPKK信號(hào)通路參與調(diào)控細(xì)胞DNA損傷修復(fù). |
| 產(chǎn)品圖片 |
Hela (H) cells were treated with or without Nocodazole, 25 μg total protein per lane of cell lysates (see on figure) probed with Phospho-PBK (Thr9) monoclonal antibody, unconjugated (bsm-62741R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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