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KDM1/LSD1 Recombinant Rabbit mAb (bsm-62859R)  
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產(chǎn)品編號(hào) bsm-62859R
英文名稱(chēng) KDM1/LSD1 Recombinant Rabbit mAb
中文名稱(chēng) 組蛋白賴(lài)氨酸特異性脫甲基酶1重組兔單抗
別    名 AOF2; CPRF; EC1; KDM1; Kdm1a; LSD1.  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Recombinant
克 隆 號(hào)
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,Flow-Cyt=1:20-50,ICC/IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 93 kDa
檢測(cè)分子量 110
性    狀 Liquid
免 疫 原 A synthesized peptide derived from human KDM1: 780-852/852 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
保存條件 Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.

SWISS:
O60341

Gene ID:
23028

產(chǎn)品圖片
Western blot analysis of HeLa cell lysate. Using KDM1/LSD1 (bsm-62859R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed MCF-7 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (KDM1/LSD1) monoclonal Antibody, unconjugated (bsm-62859R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-40295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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