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Lamin A/C Recombinant Rabbit mAb (bsm-62886R)  
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25ul/800.00元
50ul/1400.00元
100ul/2500.00元
大包裝/詢價(jià)

產(chǎn)品編號(hào) bsm-62886R
英文名稱 Lamin A/C Recombinant Rabbit mAb
中文名稱 核纖層蛋白A重組兔單抗
別    名 70 kDa lamin; LAMA, ; LMN1; LMN C; lamin A/C; Lamin; HGPS; EMD2.  
抗體來(lái)源 Rabbit
克隆類型 Recombinant
克 隆 號(hào) 8H20
交叉反應(yīng) Human
產(chǎn)品應(yīng)用 WB=1:500-5000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200,Flow-Cyt=1ug/Test,ICC/IF=1:50-200,IP=1:20-50
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 74 kDa
檢測(cè)分子量 63,74
性    狀 Liquid
免 疫 原 A synthesized peptide derived from human Lamin A/C: 520-584 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
保存條件 Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane.

SWISS:
P02545

Gene ID:
4000

核膜標(biāo)志物(Nuclear Envelope Marker) 核纖層蛋白(lamin) 是緊貼核內(nèi)膜的一層厚度為20~50nm的纖維蛋白層或纖維網(wǎng)絡(luò)。核纖層與細(xì)胞質(zhì)骨架、核骨架連成一個(gè)整體,一般認(rèn)為核纖層將核被膜和染色質(zhì)提供了結(jié)構(gòu)支架。有學(xué)者研究認(rèn)為:lamin蛋白與細(xì)胞凋亡及衰老有關(guān)聯(lián),它包括:核纖層蛋白A、核纖層蛋白B、核纖層蛋白C幾個(gè)不同亞型的蛋白。
產(chǎn)品圖片
25 ug total protein per lane of various lysates (see on figure) probed with Lamin A/C monoclonal antibody, unconjugated (bsm-62886R) at 1:2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Lamin A/C) monoclonal Antibody, unconjugated (bsm-62886R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-Lamin A/C antibody (bsm-62886R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-60295G-FITC): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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