| 產(chǎn)品編號 | bsm-62980R |
| 英文名稱 | Phospho-SMC1 alpha (Ser957) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化染色體結(jié)構(gòu)維持蛋白質(zhì)１α重組兔單抗 |
| 別 名 | SB1.8/DXS423E protein; SM1A; SMC1A; SMC1L1; SMC1alpha protein; Sb1.8 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:1000-1:2000,IHC-P=1:100-1:200,IHC-F=1:100-1:200,IF=1:50-1:200,ICC/IF=1:50-1:200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 143 kDa |
| 檢測分子量 | 160 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human SMC1 alpha around the phosphorylation site of S957: EGS-pS-QG(全長1233aa) |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. SWISS: Q8NDV3 Gene ID: 8243 |
| 產(chǎn)品圖片 |
Western blot analysis of (1) HeLa cell lysate; (2) Hela cells lysate treated with AP. Using Phospho-SMC1 alpha (Ser957) (bsm-62980R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
