| 產(chǎn)品編號 | bsm-62988R |
| 英文名稱 | Tbx21 Recombinant Rabbit mAb |
| 中文名稱 | T細胞介導的轉(zhuǎn)錄調(diào)節(jié)因子Tbx21重組兔單抗 |
| 別 名 | T bet; T box 21; T PET; TBET; TBLYM; Tbx21; TPET; Transcription factor TBLYM. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 19E12 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:200-1000,Flow-Cyt=1:20-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 58 kDa |
| 檢測分子量 | 58 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human TBX21: 500-535/535 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs.Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3. Induces permissive chromatin accessibilty and CpG methylation in IFNG. SWISS: Q9UL17 Gene ID: 30009 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Tbx21 monoclonal antibody, unconjugated (bsm-62988R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
