| 產(chǎn)品編號 | bsm-63017R |
| 英文名稱 | VASP Recombinant Rabbit mAb |
| 中文名稱 | 血管擴張刺激磷蛋白重組兔單抗 |
| 別 名 | Vasodilator-stimulated phosphoprotein; VASP. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:1000-1:2000,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 40 kDa |
| 檢測分子量 | 46 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human VASP: 44-94 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. SWISS: P50552 Gene ID: 7408 ????VASP屬于Ena-VASP蛋白家族，是一種肌動蛋白結(jié)合蛋白。細胞骨架動力學的調(diào)節(jié)在細胞粘附、細胞變形、細胞移動等生理過程中是必需的。VASP可能也參與細胞內(nèi)信號通道，該通道調(diào)節(jié)整聯(lián)蛋白與細胞外基質(zhì)間的相互作用。VASP被周期核苷酸依賴型激酶PKA與PKG所調(diào)節(jié),在一些腫瘤的分化、增值、轉(zhuǎn)移中起到一定的作用，在腫瘤中有較高的表達。 ????近年來的研究發(fā)現(xiàn)VASP在與細胞骨架調(diào)節(jié)有關(guān)的各種細胞行為中起著重要作用，如神經(jīng)細胞軸索的延伸、T細胞的移動、成纖維細胞的遷移等。 ????VAS |
| 產(chǎn)品圖片 |
Western blot analysis of HepG2 cell lysate. Using VASP (bsm-63017R) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
