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CHI3L1 Rabbit pAb (bs-1093R)  
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產(chǎn)品編號(hào) bs-1093R
英文名稱(chēng) CHI3L1 Rabbit pAb
中文名稱(chēng) 軟骨糖蛋白39抗體
別    名 CHI3L1; 39 kDa synovial protein; ASRT7; Cartilage glycoprotein 39; CGP39; chitinase 3 like 1(cartilage glycoprotein 39); chitinase 3 like 1; Chitinase 3 like protein 1 precursor; chitinase; GP 39; GP39; HC gp39; HCGP 3P; HCgp39; YKL 40; YKL40; CGP-39; CH3L1_HUMAN; chitinase 3 like 1; Chitinase-3-like protein 1 hCGP-39; YKL-40; YYL 40.  
Specific References  (4)     |     bs-1093R has been referenced in 4 publications.
[IF=16.494] Wang et al. Clara cell 10-kD protein suppresses chitinase 3-like 1 expression associated with eosinophilic chronic rhinosinusitis. (2010) Am.J.Respir.Crit.Care.Med. 181:908-16  IHC ;  Mouse&Human.  
[IF=3.41] Tanaka, Yuki, et al. "Antigen‐specific overexpression of human cartilage glycoprotein 39 on CD4+ CD25+ Foxp3+ Treg cells in the generation of glucose‐6‐phosphate isomerase induced arthritis." Clinical & Experimental Immunology (2014).  Mouse.  
[IF=3.265] Zheng et al. Establishment of using serum YKL-40 and SCCA in combination for the diagnosis of patients with esophageal squamous cell carcinoma. (2014) BMC.Cance. 14:490  WB,IHC-P ;  Human.  
[IF=1.29] Ben, Su-qin, et al. "Ovalbumin enhances YKL-40, IL-5, GM-CSF, and eotaxin expression simultaneously in primarily cultured mouse tracheal epithelial cells." In Vitro Cellular & Developmental Biology-Animal (2013): 1-8.  WB ;  Mouse.  
研究領(lǐng)域 細(xì)胞生物  信號(hào)轉(zhuǎn)導(dǎo)  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-2000,ICC/IF=1:100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 42 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞外基質(zhì) 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from rat CHI3L1: 330-381/381 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009]

Function:
Carbohydrate-binding lectin with a preference for chitin. May play a role in defense against pathogens, or in tissue remodeling. May play an important role in the capacity of cells to respond to and cope with changes in their environment.

Subunit:
Monomer.

Subcellular Location:
Secreted, extracellular space.

Tissue Specificity:
Present in articular chondrocytes, synovial cells as well as in liver. Undetectable in muscle tissues, lung, pancreas, mononuclear cells, or fibroblasts.

Similarity:
Belongs to the glycosyl hydrolase 18 family.

SWISS:
Q9WTV1

Gene ID:
89824

Database links:

Entrez Gene: 1116 Human

Entrez Gene: 12654 Mouse

Entrez Gene: 396865 Pig

Entrez Gene: 89824 Rat

Omim: 601525 Human

SwissProt: P36222 Human

SwissProt: Q61362 Mouse

SwissProt: Q5RBP6 Orangutan

SwissProt: Q29411 Pig

SwissProt: Q9WTV1 Rat

Unigene: 382202 Human

Unigene: 38274 Mouse

Unigene: 68940 Rat



產(chǎn)品圖片
Protein: Liver(Rat) lysate at 30ug; Colon carcinoma(Human) lysates at 30ug; Primary: Anti-CGP-39/CHI3L1/YKL-40 (bs-1093R) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; Predicted band size : 42kD Observed band size : 39kD
Sample: Lane 1: Mouse Lung tissue lysates Lane 2: Rat Lung tissue lysates Primary: Anti-CHI3L1 (bs-1093R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa
U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (CHI3L1) polyclonal Antibody, Unconjugated (bs-1093R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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