| 產品編號 | bs-2437R |
| 英文名稱 | ENPP2 Rabbit pAb |
| 中文名稱 | 自分泌運動因子抗體 |
| 別 名 | Autotaxin; Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; ATX; ATX X; ATX-X; Autotaxin; E NPP 2; ENPP2; Ectonucleotide pyrophosphatase/phosphodiesterase 2; Extracellular lysophospholipase D; FLJ26803; LysoPLD; NPP2; PD IALPHA; PD-IALPHA; PDNP2; phosphodiesterase I alpha; phosphodiesterase I/nucleotide pyrophosphatase 2; Plasma lysophospholipase D; ENPP2_HUMAN. |
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Specific References (1) | bs-2437R has been referenced in 1 publications.
[IF=3.934] Wang Z et al. Fetal Bisphenol-A Induced Changes in Murine Behavior and Brain Gene Expression Persisted in Adult-aged OffspringEndocrinology.2020 Dec 1;161(12):bqaa164. IHC ; Mouse.
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| 研究領域 | 腫瘤 細胞生物 免疫學 神經生物學 信號轉導 細胞凋亡 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 95 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ENPP2: 601-700/915 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq] Function: Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor. Subcellular Location: Secreted. Note=Secreted by most body fluids including serum and CSF. Also by adipocytes and numerous cancer cells. Tissue Specificity: Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues. Post-translational modifications: N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity. It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both. Similarity: Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. Contains 2 SMB (somatomedin-B) domains. SWISS: Q13822 Gene ID: 5168 Database links: Entrez Gene: 5168 Human Entrez Gene: 18606 Mouse Omim: 601060 Human SwissProt: Q13822 Human SwissProt: Q9R1E6 Mouse Unigene: 190977 Human Unigene: 250256 Mouse ATX是一個分泌型糖蛋白,具有磷酸二酯酶（PDE）活性，ATX是胞外焦磷酸酶／磷酸二酯酶（ENPP）家族的一員。ATX還具有溶血磷脂酶D（lysoPLD）活性，能夠以溶血磷脂酰膽堿（lysophosphatidylcboline，LPC）為底物催化生成溶血磷脂酸（lysophosphatidic acid，LPA）。 ATX在很多腫瘤細胞中都有高表達，在腫瘤的發(fā)生、發(fā)展過程中有著重要作用。此外，ATX在神經系統(tǒng)、免疫系統(tǒng)中也發(fā)揮重要作用。 |
| 產品圖片 |
Sample:
DU145(Human) Cell Lysate at 30 ug
Primary: Anti-Anei-ENPP2 (bs-2437R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kD
Observed band size: 115 kD
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Anei-ATX/Autotaxin Polyclonal Antibody, Unconjugated(bs-2437R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control（black line）:Hela.
Primary Antibody (green line): Rabbit Anti-ENPP2 antibody (bs-2437R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
