| 產(chǎn)品編號 | bs-2531R |
| 英文名稱 | NES/Kallikrein 10 Rabbit pAb |
| 中文名稱 | 上皮細胞特異性蛋白1抗體 |
| 別 名 | Kallikrein 10; Breast normal epithelial cell associated serine protease; Kallikrein related peptidase 10; KLK 10; KLK10; KLK10_HUMAN; NES 1; NES1; Normal epithelial cell specific 1; Protease serine like 1; PRSS L1; PRSSL 1; PRSSL1. |
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Specific References (3) | bs-2531R has been referenced in 3 publications.
[IF=2.559] Cao XY et al. Aberrant upregulation of KLK10 promotes metastasis via enhancement of EMT and FAK/SRC/ERK axis in PDAC.Biochem Biophys Res Commun. 2018 May 15;499(3):584-593. IHC-P&WB ; Mouse&Human.
[IF=2.3] Li, Lei, et al. "Upregulated KLK10 inhibits esophageal cancer proliferation and enhances cisplatin sensitivity in vitro." Oncology Reports. WB ;
[IF=0] Wang et al. Chemoprevention activity of dipyridamole in the MMTV-PyMT transgenic mouse model of breast cancer. (2013) Cancer.Prev.Res.(Phila. 6:437-47 WB ; Human.
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| 研究領域 | 腫瘤 細胞生物 信號轉導 激酶和磷酸酶 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human (predicted: Mouse,Rat,Cow) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 31 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KLK10: 65-165/276 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Kallikrein 10 is a trypsin-like serine proteinase. Originally described from a subtractive library looking for genes that were down-regulated in breast cancer, kallikrein 10 was thought to be epithelial cell specific. Kallikrein 10 is reported to be up regulated in pancreatic cancer and ovarian cancer, but down regulated in testicular cancer and breast cancer, perhaps due to differential hormonal regulation, or tissue specificities. Breast cancer patients that had elevated kallikrein 10 had a worse prognosis, leading to speculation that kallikrein 10 might be protective in breast cancer, similar to the argument put forth for kallikrein 3 in prostate cancer. Kallikrein 10 is found in high levels in the pancreas and in lower levels in the tonsils, esophagus, skin, brain, testis and ovary. Biological fluids (breast milk, serum, seminal plasma, urine) contain kallikrein 10, the highest levels found in milk. The cerebral spinal fluid (CSF) contains Kallikrein 10, and was reported to be elevated in patients with Alzheimer’s disease. Endogenous inhibitors include kallistatin, protein-C inhibitor and 1-proteinase inhibitor, although Kallikrein 10 can be found complexed to a number of different proteinase inhibitors. Function: Has a tumor-suppressor role for NES1 in breast and prostate cancer. Subcellular Location: Secreted. Tissue Specificity: Expressed in breast, ovary and prostate. Similarity: Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain. SWISS: O43240 Gene ID: 5655 Database links: Entrez Gene: 5655 Human Omim: 602673 Human SwissProt: O43240 Human Unigene: 275464 Human KLK10是組織激肽釋放酶基因家族的重要成員,是當前研究較熱的腫瘤生物學標志物之一,在多種與內分泌相關的腫瘤中有較特異性的表達。 |
| 產(chǎn)品圖片 |
Sample:
DU145(Human) Cell Lysate at 30 ug
Primary: Anti-NES/Kallikrein 10 (bs-2531R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Sample:
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti-NES/Kallikrein 10 (bs-2531R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
