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Cardiac Troponin T Rabbit pAb (bs-2804R)  
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產品編號 bs-2804R
英文名稱 Cardiac Troponin T Rabbit pAb
中文名稱 心肌特異性肌鈣蛋白T抗體
別    名 Cardiac muscle troponin T; Cardiomyopathy dilated 1D(autosomal dominant); Cardiomyopathy hypertrophic 2; CMD1D; CMH2; CMPD2; cTnT; LVNC6; MGC3889; OTTHUMP00000033864; OTTHUMP00000033865; OTTHUMP00000033866; OTTHUMP00000033867; OTTHUMP00000033870; OTTHUMP00000218095; RCM3; TNNT 2; TNNT2; TNNT2_HUMAN; TnTC; Troponin T cardiac muscle; Troponin T type 2(cardiac); Troponin T type 2 cardiac; Troponin T, cardiac muscle; Troponin T2; Troponin T2 cardiac.  
Specific References  (2)     |     bs-2804R has been referenced in 2 publications.
[IF=3.612] Zhou et al. Rapid and Efficient Generation of Transgene-Free iPSC from a Small Volume of Cryopreserved Blood. (2015) Stem.Cell.Rev. 11:652-65  IHC ;  Human,?Mouse,?Rat,?Dog,?Pig,?Cow,?Rabbit,?Guinea Pig.  
[IF=3.065] Li M et al. Aconitine induces cardiotoxicity through regulation of calcium signaling pathway in zebrafish embryos and in H9c2 cells. J Appl Toxicol. 2020 Jan 23.  WB ;  Rat.  
研究領域 心血管  免疫學  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human (predicted: Mouse,Rat,Rabbit,Pig,Cow,Dog,GuineaPig)
產品應用 Flow-Cyt=1μg /Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 36 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Troponin T, cardiac muscle.: 201-298/298 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq].

Function:
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.

DISEASE:
Cardiomyopathy, familial hypertrophic 2 (CMH2) [MIM:115195]: A hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. Note=The disease is caused by mutations affecting the gene represented in this entry.
Cardiomyopathy, dilated 1D (CMD1D) [MIM:601494]: A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Note=The disease is caused by mutations affecting the gene represented in this entry.
Cardiomyopathy, familial restrictive 3 (RCM3) [MIM:612422]: A heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the troponin T family.

SWISS:
P45379

Gene ID:
7139

Database links:

Entrez Gene: 493940 Cat

Entrez Gene: 286816 Cow

Entrez Gene: 403532 Dog

Entrez Gene: 7139 Human

Entrez Gene: 21956 Mouse

Entrez Gene: 100622450 Pig

Entrez Gene: 100009428 Rabbit

Entrez Gene: 24837 Rat

Omim: 191045 Human

SwissProt: P13789 Cow

SwissProt: P45379 Human

SwissProt: P50752 Mouse

SwissProt: P09741 Rabbit

SwissProt: P50753 Rat

Unigene: 533613 Human

Unigene: 247470 Mouse

Unigene: 9965 Rat



產品圖片
Blank control:U-2OS. Primary Antibody (green line): Rabbit Anti-TNNT2 antibody (bs-2804R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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