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SLU7 Rabbit pAb (bs-8327R)  
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產(chǎn)品編號(hào) bs-8327R
英文名稱 SLU7 Rabbit pAb
中文名稱 SLU7蛋白抗體
別    名 9G8; hSlu7; MGC9280; Pre mRNA splicing factor SLU7; Pre-mRNA-splicing factor slu7; SLU 7; SLU-7; slu7; SLU7 splicing factor homolog(S. cerevisiae); SLU7 splicing factor homolog; SLU7_HUMAN; Step II splicing factor SLU7; Zinc knuckle motif containing.  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  表觀遺傳學(xué)  
抗體來(lái)源 Rabbit
克隆類(lèi)型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Rat
產(chǎn)品應(yīng)用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 68 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SLU7: 301-400/586 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Participates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'-splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region. May be involved in the activation of proximal AG. Probably also involved in alternative splicing regulation.

Function:
Participates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'-splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region. May be involved in the activation of proximal AG. Probably also involved in alternative splicing regulation.

Subunit:
Component of late spliceosomal complexes. Associates with the spliceosome prior to recognition of the 3'-splice site for step II, probably during catalysis of step I.

Subcellular Location:
Nucleus. Cytoplasm. Predominantly nuclear. Shuttling between the nucleus and the cytoplasm is regulated by the CCHC-type zinc finger. Upon UV-C stress stimulus, the nuclear concentration of the protein decreases, affecting alternative splicing.

Similarity:
Belongs to the SLU7 family.

SWISS:
O95391

Gene ID:
10569

Database links:

Entrez Gene: 10569 Human

Entrez Gene: 193116 Mouse

Entrez Gene: 303057 Rat

Omim: 605974 Human

SwissProt: O95391 Human

SwissProt: Q8BHJ9 Mouse

SwissProt: Q80ZG5 Rat

Unigene: 435342 Human

Unigene: 28200 Mouse

Unigene: 137183 Rat



產(chǎn)品圖片
25 ug total protein per lane of various lysates (see on figure) probed with SLU7 polyclonal antibody, unconjugated (bs-8327R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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