| 產品編號 | bs-14067R |
| 英文名稱 | CROP Rabbit pAb |
| 中文名稱 | 環(huán)磷酸腺苷調節(jié)元件相關蛋白1抗體 |
| 別 名 | cAMP regulatory element-associated protein 1; Cisplatin resistance-associated overexpressed protein; Cisplatin resistance-associated-overexpressed protein; CRE-associated protein 1; CREAP-1; CREAP1; CROP; LC7L3_HUMAN; Luc7-like protein 3; Luc7A; LUC7L3; O48; Okadaic acid-inducible phosphoprotein OA48-18. |
| 研究領域 | 細胞生物 轉錄調節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Chicken) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 51 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CROP: 31-130/432 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
This gene encodes a protein with an N-terminal half that contains cysteine/histidine motifs and leucine zipper-like repeats, and the C-terminal half is rich in arginine and glutamate residues (RE domain) and arginine and serine residues (RS domain). This protein localizes with a speckled pattern in the nucleus, and could be involved in the formation of splicesome via the RE and RS domains. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2009] Function: Binds cAMP regulatory element DNA sequence. May play a role in RNA splicing. Subcellular Location: Nucleus speckle. The subnuclear localization is affected by cisplatin. Tissue Specificity: Widely expressed. Highest levels in heart, brain, pancreas, thymus, ovary, small intestine and peripheral blood leukocytes, as well as cerebellum, putamen and pituitary gland. Lowest levels in lung, liver and kidney. Also expressed in fetal tissues, including brain, heart, kidney, thymus and lung. Post-translational modifications: Phosphorylated in vitro by SRPK1, SRPK2 and CLK1. Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the Luc7 family. SWISS: O95232 Gene ID: 51747 Database links: Entrez Gene: 51747 Human Entrez Gene: 67684 Mouse Omim: 609434 Human SwissProt: O95232 Human SwissProt: Q5SUF2 Mouse Unigene: 130293 Human Unigene: 30927 Mouse |
| 產品圖片 |
Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti- CROP (bs-14067R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 53 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CROP Polyclonal Antibody, Unconjugated(bs-14067R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
