| 產(chǎn)品編號 | bs-14320R |
| 英文名稱 | DHX37 Rabbit pAb |
| 中文名稱 | DHX37蛋白抗體 |
| 別 名 | DDX37; DEAH(Asp-Glu-Ala-His) box polypeptide 37; DEAH box protein 37; FLJ41974; Gm1050; DHX37_HUMAN; Gm451; KIAA1517; MGC2695; MGC4322; MGC46245; MGC90028; mKIAA1517; Probable ATP dependent RNA helicase DHX37. |
| 研究領(lǐng)域 | 細胞生物 發(fā)育生物學 細胞分化 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Rat (predicted: Mouse,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 129 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human DHX37: 51-150/1157 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a DEAD box protein. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. [provided by RefSeq, Jul 2008] Function: DHX37 is a member of the family of the DEAD box proteins, a group of proteins that present the conserved motif Asp-Glu-Ala-Asp (DEAD). They have a role in RNA translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly Similarity: Belongs to the DEAD box helicase family. DEAH subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. SWISS: Q8IY37 Gene ID: 57647 Database links: Entrez Gene: 57647 Human Entrez Gene: 208144 Mouse SwissProt: Q8IY37 Human |
| 產(chǎn)品圖片 |
Sample: 293T Cell (Human) Lysate at 40 ug
Primary: Anti-DHX37(bs-14320R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 129kD
Observed band size: 135kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DHX37) Polyclonal Antibody, Unconjugated (bs-14320R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
