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MOCS3 Rabbit pAb (bs-17702R)  
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產品編號 bs-17702R
英文名稱 MOCS3 Rabbit pAb
中文名稱 鉬輔因子合成蛋白3抗體
別    名 Adenylyltransferase MOCS3; dJ914P20.3; MGC9252; MOCS 3; MOCS3; MOCS3_HUMAN; Molybdenum cofactor synthesis 3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; Molybdopterin-synthase sulfurtransferase; MPT synthase sulfurylase; Sulfur carrier protein MOCS2A adenylyltransferase; Sulfur carrier protein MOCS2A sulfurtransferase; Sulfurtransferase MOCS3; UBA 4; UBA4 ubiquitin activating enzyme E1 homolog; UBA4.  
研究領域 細胞生物  信號轉導  激酶和磷酸酶  新陳代謝  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human (predicted: Mouse)
產品應用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 50 kDa
檢測分子量
細胞定位 細胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human MOCS3: 11-110/460 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 Molybdenum cofactor (MoCo) is necessary for the function of all molybdoenzymes. The protein encoded by this gene adenylates and activates molybdopterin synthase, an enzyme required for biosynthesis of MoCo. This gene contains no introns. A pseudogene of this gene is present on chromosome 14. [provided by RefSeq, Nov 2012]

Function:
Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.

Subunit:
Interacts with NFS1.

Subcellular Location:
Cytoplasm > cytosol.

Similarity:
In the N-terminal section; belongs to the hesA/moeB/thiF family. UBA4 subfamily. Contains 1 rhodanese domain.

SWISS:
O95396

Gene ID:
27304

Database links:

Entrez Gene: 27304 Human

Entrez Gene: 69372 Mouse

GenBank: BC015939 Human

Omim: 609277 Human

SwissProt: O95396 Human

SwissProt: A2BDX3 Mouse

Unigene: 159410 Human

Unigene: 482182 Mouse



產品圖片
Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-MOCS3 (bs-17702R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD
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