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CH25H Rabbit pAb (bs-23620R)  
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產品編號 bs-23620R
英文名稱 CH25H Rabbit pAb
中文名稱 膽固醇25羥化酶抗體
別    名 C25H; Cholesterol 25 hydroxylase; Cholesterol 25-hydroxylase; Cholesterol 25 monooxygenase; EC 1.14.99.38; EC=1.14.99.38; h25OH; CH25H_HUMAN.  
研究領域 腫瘤  細胞生物  信號轉導  激酶和磷酸酶  新陳代謝  
抗體來源 Rabbit
克隆類型 Polyclonal
克 隆 號
交叉反應 Human
產品應用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 32 kDa
檢測分子量
細胞定位 細胞漿 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CH25H : 181-272/272 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產品介紹 CH25H (Cholesterol 25-hydroxylase), also known as h25OH, is a 272 amino acid endoplasmic membrane protein that belongs to the sterol desaturase family. CH25H contains clusters of histidine residues essential for catalytic activity and is involved in cholesterol and lipid metabolism. CH25H catalyzes the formation of 25-hydroxycholesterol from cholesterol leading to the repression of cholesterol biosynthetic enzymes. CH25H regulates lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. CH25H utilizes diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.

Function:
CH25H is involved in cholesterol and lipid metabolism and catalyses the formation of 25-hydroxycholesterol from cholesterol, leading to repression of cholesterol biosynthetic enzymes. It may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing.

Subcellular Location:
Endoplasmic reticulum membrane

Post-translational modifications:
N-glycosylated.

Similarity:
Belongs to the sterol desaturase family.

SWISS:
O95992

Gene ID:
9023

Database links:

Entrez Gene: 9023 Human

Entrez Gene: 12642 Mouse

Entrez Gene: 309527 Rat

Omim: 604551 Human

SwissProt: O95992 Human

SwissProt: Q9Z0F5 Mouse

SwissProt: Q4QQV7 Rat



產品圖片
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CH25H) Polyclonal Antibody, Unconjugated (bs-23620R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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