| 產(chǎn)品編號(hào) | bsm-60597R |
| 英文名稱 | TG Recombinant Rabbit mAb |
| 中文名稱 | 甲狀腺球蛋白重組兔單抗 |
| 別 名 | THYG_HUMAN; Thyroglobulin; AITD 3; AITD3; hTG; TDH3; Tgn; |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號(hào) | A7B9 |
| 交叉反應(yīng) | Human,Mouse,Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:50-200,IHC-F=1:50-200,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 660 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | A synthesized peptide derived from human Thyroglobulin: 1001-1023/2768 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis. [provided by RefSeq, Nov 2009] SWISS: P01266 Gene ID: 7038 Database links: Entrez Gene: 7038 Human SwissProt: P01266 Human 用作生產(chǎn)碘化甲狀腺激素甲狀腺素（T4）和三碘甲狀腺素（T3）的底物。 T3和T4的合成涉及對(duì)TG /甲狀腺球蛋白的選定酪氨酸殘基加碘，然后在甲狀腺濾泡內(nèi)腔中進(jìn)行氧化偶聯(lián)。 在TG重新內(nèi)在化和溶酶體介導(dǎo)的蛋白水解之后，T3和T4從多肽主鏈釋放，導(dǎo)致它們分泌到血液中。 一個(gè)二聚體產(chǎn)生7個(gè)甲狀腺激素分子。 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with TG monoclonal antibody, unconjugated (bsm-60597R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Thyroid Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TG Monoclonal Antibody, Unconjugated(bsm-60597R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TG Monoclonal Antibody, Unconjugated(bsm-60597R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Thyroid Gland; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with TG Monoclonal Antibody, Unconjugated(bsm-60597R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
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