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PTBP1 Recombinant Rabbit mAb (bsm-60550R)  
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25ul/800.00元
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產(chǎn)品編號(hào) bsm-60550R
英文名稱 PTBP1 Recombinant Rabbit mAb
中文名稱
抗體來源 Rabbit
克隆類型 Recombinant
克 隆 號(hào) C1F6
交叉反應(yīng) Human (predicted: Mouse)
產(chǎn)品應(yīng)用 WB=1:500-1:1000,Flow-Cyt=1:50-100,ICC/IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
亞    型 IgG/Kappa
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 在mRNA前體剪接和選擇性剪接事件的調(diào)控中發(fā)揮作用。在肌肉細(xì)胞分化過程中激活自身前體mRNA的外顯子跳躍。與內(nèi)含子的多嘧啶束結(jié)合。當(dāng)與同一前體mRNA中的兩個(gè)單獨(dú)的多嘧啶束結(jié)合時(shí),可能促進(jìn)RNA循環(huán)??赡艽龠M(jìn)U2-snRNP與premrna的結(jié)合。在TPM1前體mRNA成熟過程中與RAVER1協(xié)同調(diào)節(jié)相互排斥的外顯子之間的轉(zhuǎn)換。抑制MAPT/Tau外顯子10的剪接。在小核糖核酸病毒感染的情況下,與病毒內(nèi)部核糖體進(jìn)入位點(diǎn)(IRES)結(jié)合并刺激IRES介導(dǎo)的翻譯。
產(chǎn)品圖片
Blocking buffer: 5% NFDM/TBST Primary ab dilution: 1:1000 Primary ab incubation condition: 2 hours at room temperature Secondary ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: HeLa, Ramos, 4T1 Protein loading quantity: 20 μg Exposure time: 30 s Predicted MW: 57 kDa Observed MW: 57 kDa
4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (PTBP1) monoclonal Antibody, unconjugated (bsm-60550R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Rabbit Anti-PTBP1 antibody (bsm-60550R,1:100); Secondary Antibody (white blue): Goat anti-Rabbit IgG-BF488 (bs-60295G-BF488): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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