產(chǎn)品編號 | bsm-60565R |
英文名稱 | PDP1 Recombinant Rabbit mAb |
中文名稱 | |
抗體來源 | Rabbit |
克隆類型 | Recombinant |
克 隆 號 | F12B3 |
交叉反應 | Human (predicted: Mouse,Rat) |
產(chǎn)品應用 | WB=1:500-1:1000,ICC/IF=1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理論分子量 | kDa |
檢測分子量 | |
細胞定位 | 細胞膜 |
性 狀 | Liquid |
濃 度 | 1mg/ml |
亞 型 | IgG/Kappa |
純化方法 | affinity purified by Protein A |
緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
產(chǎn)品介紹 | 催化丙酮酸脫氫酶復合物E1組分的α亞單位的去磷酸化和伴隨的再活化。 |
產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: U87MG, SK-OV-3, PC-3, 3T3
Protein loading quantity: 20 μg
Exposure time: 60 s
Predicted MW: 55 kDa
Observed MW: 55 kDa
|
1、抗體溶解方法 | |
2、抗體修復方式 | |
3、常用試劑的配制 | |
4、免疫組化操作步驟 | |
5、免疫組化問題解答 | |
6、Western Blotting 操作步驟 | |
7、Western Blotting 問題解答 | |
8、關(guān)于肽鏈的設計 | |
9、多肽的溶解與保存 | |
10、酶標抗體效價測定程序 | |