| 產(chǎn)品編號 | bsm-60559M |
| 英文名稱 | CHEK2 Mouse mAb |
| 中文名稱 | 細胞周期檢測點激酶2單克隆抗體 |
| 別 名 | CHK2_HUMAN; Serine/threonine-protein kinase Chk2; EC:2.7.11.1; Serine/threonine-protein kinase Chk2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425; CHK2 checkpoint homolog; Cds1 homolog(Hucds1; hCds1); Checkpoint kinase 2; |
| 抗體來源 | Mouse |
| 克隆類型 | Monoclonal |
| 克 隆 號 | E3E7 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-1:1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 56 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG2B/lambda |
| 純化方法 | affinity purified by Protein G |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] Subcellular Location: Isoform 2: Nucleus. Note=Isoform 10 is present throughout the cell. Isoform 4: Nucleus. Isoform 7: Nucleus. Isoform 9: Nucleus. Isoform 12: Nucleus. Nucleus, PML body. Nucleus, nucleoplasm. Note=Recruited into PML bodies together with TP53. Tissue Specificity: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues. Database links: Entrez Gene: 11200 Human SwissProt: O96017 Human 絲氨酸/蘇氨酸蛋白激酶，是檢測點介導(dǎo)的細胞周期阻滯、DNA修復(fù)激活和DNA雙鏈斷裂引起的細胞凋亡所必需的。在未受干擾的細胞周期中也可能負性調(diào)節(jié)細胞周期進程。活化后，在共有序列[L-X-R-X-X-S/T]上優(yōu)先磷酸化許多效應(yīng)子。通過CDC25A、CDC25B和CDC25C的磷酸化調(diào)節(jié)細胞周期檢查點阻滯，抑制其活性。CDC25磷酸酶活性的抑制導(dǎo)致CDK-細胞周期蛋白復(fù)合物酪氨酸磷酸化的抑制性增加并阻斷細胞周期進程。也可能磷酸化參與G2/M細胞周期阻滯的NEK6。通過BRCA2的磷酸化調(diào)節(jié)DNA修復(fù)，增強RAD51與染色質(zhì)的結(jié)合，通過同源重組促進DNA修復(fù)。還通過轉(zhuǎn)錄因子FOXM1的磷酸化和激活刺激參與DNA修復(fù)的基因（包括BRCA2）的轉(zhuǎn)錄。 |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: room temperature 2h
Secondary ab: Goat Anti-Mouse IgG H&L (HRP)
Lysate: HeLa, HCT-116, MOLT-4
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 62 kDa
Observed MW: 62 kDa
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
