| 產(chǎn)品編號 | bsm-60706R |
| 英文名稱 | Alpha Tubulin (Acetyl-Lys40) Recombinant Rabbit mAb |
| 中文名稱 | 乙酰化微管蛋白α/Tubulin α重組兔單抗 |
| 別 名 | alpha tubulin 1; alpha-tubulin 1; Detyrosinated alpha Tubulin; FLJ30169; H2 alpha; TBA4A_HUMAN; TBA1A_HUMAN; TBA1B_HUMAN; Testis specific alpha tubulin; Testis-specific alpha-tubulin; TUBA 4A; TUBA1; Tuba4a; Tubulin alpha 1(testis specific); Tubulin alpha |
| 產(chǎn)品類型 | 乙?；贵w 重組兔單抗 |
| 研究領域 | 細胞生物 染色質(zhì)和核信號 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | R2A9 |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:1000-4000,IHC-P=1:100-500,IHC-F=1:50-100,IF=1:25,ICC/IF=1:25
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 50 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Alpha Tubulin (Acetyl-Lys40): SD(Ac-K)TI |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013] Subcellular Location: Cytoplasm, cytoskeleton. Tissue Specificity: Widely expressed. Overexpressed in Platelet, Brain, and Frontal cortex SWISS: P68366 Gene ID: 7277 Database links: Entrez Gene: 7277 Human Entrez Gene: 22145 Mouse SwissProt: P68366 Human SwissProt: P68368 Mouse |
| 產(chǎn)品圖片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:6000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: 1: Mouse brain, 2: Rat brain
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 50 kDa
Observed MW: 55 kDa
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:1000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: (-): MCF-7+serum starvation(14hr), (+):
MCF-7+ sodium butyrate (50mM, 24hr) +
trichostain A (500ng/ml, 4 hr)
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 50 kDa
Observed MW: 55 kDa
Tissue: Rat cerebellum
Section type: Formalin-fixed & Paraffin
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal
for
bsm-60706R
Tissue: Human cerebrum
Section type: Formalin-fixed & Paraffin
embedded section
Retrieval method: High temperature and high
pressure
Retrieval buffer: Tris/EDTA buffer, pH 9.0
Primary Ab dilution: 1:100
Primary Ab incubation condition: 1 hour at
room temperature
Secondary Ab: Anti-Rabbit and Mouse
Polymer HRP (Ready to use)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal
for bsm-60706R
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
