| 產(chǎn)品編號 | IHC0123H |
| 英文名稱 | Human PCNA Ready-To-Use IHC Kit |
| 中文名稱 | 人增殖細胞核抗原即用型免疫組化試劑盒 |
| 性 狀 | Liquid |
| 種 屬 | Human |
| 應用范圍 | Immunohistochemistry |
| 適用樣品基質(zhì) | FFPE tissue |
| 描述 | IHC0123H is a ready-to-use IHC kit for staining of human PCNA. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data. Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose ap |
| 保存條件 | Please store components at the temperatures indicated on the individual tube labels. The kit is stable for 6 months from the date of receipt. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產(chǎn)品介紹 |
Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV41) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure. SWISS: P12004 Gene ID: 5111 |
| 產(chǎn)品圖片 |
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Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue slide using IHC0123H (Human PCNA IHC Kit).
Immunohistochemical analysis of paraffin embedded human liver carcinoma tissue slide using IHC0123H (Human PCNA IHC Kit).
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
