| 產(chǎn)品編號 | bs-42347R |
| 英文名稱 | Wnt11 Rabbit pAb |
| 中文名稱 | 信號通路Wnt11抗體 |
| 別 名 | HWNT11; Protein Wnt-11; Wingless-related MMTV integration site 11; Wingless-type MMTV integration site family, member 1; Wingless-type MMTV integration site family, member 11; Wingless-type MMTV integration site family, member 11B; wnt-11; wnt11b.WNT11_HUMAN |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:200-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞外基質(zhì) 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant mouse Wnt11 protein: 253-354/354 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The Wnt genes belong to a family of protooncogenes with at least 13 known members that are expressed in species ranging from Drosophila to man. The name Wnt denotes the relationship of this family to the Drosophila segment polarity gene ”wingless” and to its vertebrate ortholog, Int-1, a mouse proto-oncogene. Transcription of Wnt family genes appears to be developmentally regulated in a precise temporal and spatial manner. The Wnt genes encode cysteine-rich putative glycoproteins which have features typical of secreted growth factors. Wnt-11 is expressed in the tips of ureteric buds and in the perichondrium, a stem cell-like layer that surrounds the future bones and directs their growth and regeneration. Wnt-11 activity is required for cells to undergo correct convergent extension movements during gastrulation. Human Wnt-11 is also expressed in the lung mesenchyme, the urorectal septum, the urogenital folds, the labioscrotal swellings, and the cortex of the adrenal gland. Unlike other Wnt family members, Wnt-11 is not expressed in the neuroepithelium of the central nervous system. Wnt-11, along with Wnt-8c, is ex-pressed in the posterior region of the chick embryo in the caudal paraxial mesoderm that underlies the prospective caudal neural plate. The gene which encodes Wnt-11 maps to human chromosome 11q13.5. Function: Wnt11 is a non-canonical Wnt which is expressed in androgen-independent prostate cancer cell lines and prostate tumours. It is involved in development, including cardiac and gut development. Subcellular Location: Secreted; extracellular space; extracellular matrix. Tissue Specificity: Expressed in fetal lung, kidney, adult heart, liver, skeletal muscle, and pancreas. Post-translational modifications: Palmitoylation at Ser-215 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-80. Palmitoylation is necessary for proper trafficking to cell surface (By similarity). Similarity: Belongs to the Wnt family. SWISS: P48615 Gene ID: 22411 Database links: Entrez Gene: 7481 Human Entrez Gene: 22411 Mouse Omim: 603699 Human SwissProt: O96014 Human SwissProt: P48615 Mouse |
| 產(chǎn)品圖片 |
20 ng rmWnt11-Trx-His protein (bs-42347P) per lane probed with Wnt11 polyclonal antibody respectively, unconjugated (bs-42347R) at 1:1000 dilution and 4°C overnight incubation. Followed by corresponding conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with Wnt11 polyclonal antibody, unconjugated (bs-42347R) at 1:300 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
|
