| 產(chǎn)品編號 | bsm-52791R |
| 英文名稱 | MRC1 Recombinant Rabbit mAb |
| 中文名稱 | 巨噬細胞甘露糖受體 (CD206)重組兔單抗 |
| 別 名 | Mannose Receptor; C-type lectin domain family 13 member D; CD 206; CD206; CD206 antigen; CLEC13D; CLEC13DL; Macrophage mannose receptor; Mannose receptor C type 1; MMR; MRC 1; MRC-1; MRC1_HUMAN; Macrophage mannose receptor 1; MRC1L1; C-type lectin domain |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 細胞膜受體 糖蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | CD206 |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,Flow-Cyt=1ug/Test,ICC/IF=1:100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 166/140 kDa |
| 檢測分子量 | |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Macrophage mannose receptor 1: 1400-1456/1456 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The recognition of complex carbohydrate structures on glycoproteins is an important part of several biological processes, including cell-cell recognition, serum glycoprotein turnover, and neutralization of pathogens. The protein encoded by this gene is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. The protein has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. This gene is in close proximity to MRC1L1. The gene loci including this gene, MRC1L1, as well as LOC340843 and LOC340893, consist of two nearly identical, tandemly linked genomic regions, which are thought to be a part of a duplicated region. [provided by RefSeq] Function: Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens. Subcellular Location: Endosome membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Similarity: Contains 8 C-type lectin domains. Contains 1 fibronectin type-II domain. [SIMILARITY] Contains 1 ricin B-type lectin domain. SWISS: P22897 Gene ID: 4360 Database links: Entrez Gene: 4360 Human Entrez Gene: 17533 Mouse Omim: 153618 Human SwissProt: P22897 Human SwissProt: Q61830 Mouse Unigene: 75182 Human Unigene: 2019 Mouse 巨噬細胞甘露糖受體,主要存在于巨噬細胞膜表面的模式識別受體，能夠特異性識別甘露糖、巖藻糖或N-乙酰葡糖胺，介導(dǎo)巨噬細胞吞噬抗原，啟動免疫反應(yīng). |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with MRC1 monoclonal antibody, unconjugated (bsm-52791R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
4% Paraformaldehyde-fixed Molt-4 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (MRC1) monoclonal Antibody, unconjugated (bsm-52791R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Molt-4(H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Rabbit Anti-MRC1 antibody (bsm-52791R): 1 μg/10^6 cells; Secondary Antibody (white blue): Goat anti-Rabbit IgG-FITC (bs-60295G-FITC): 1 μg/test. Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
